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pfuse higg1 fc2 plasmid  (InvivoGen)


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    Structured Review

    InvivoGen pfuse higg1 fc2 plasmid
    Pfuse Higg1 Fc2 Plasmid, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfuse+higg1+fc2+plasmid/pFUSE-hIgG1-Fc2/pmc13037750-37-5-7
    Average 96 stars, based on 213 article reviews
    pfuse higg1 fc2 plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Construct:

    Article Title: NanoB 2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET
    Article Snippet: Generation of NanoLuc-tagged receptor plasmids NanoLuc-ACKR3 and NanoLuc-CXCR4 in pcDNA3.1 have been described previously.34 The pcDEF3 vector was a gift from Langer.59 The pET28a and pMEK222 vectors for periplasmic production of nanobodies in E.coli have been described previously.60,61 NanoLuc-US28 in pcDEF3 has been described previously.38 HA-US28-NanoLuc in pcDEF3 was generated by replacing BioID2 in the HA-US28-BioID2 pcDEF3 construct, which has been described previously.38 HA-CXCR4, HA-ACKR3 and HA-US28 in pcDEF3 have been described previously.62,63,64 NanoLuc-EGFR in a pNKF1-secN CMV vector was a kind gift from Promega Corporation (Wisconsin, USA), whose generation has been described previously.42. . Generation of nanobody-C-Direct and nanobody-Fc plasmids For nanobody-C-Direct constructs, VHH genes were inserted in pYQVQ11 plasmid (QVQ Holding B.V.), with a C-terminal tag containing an unpaired cysteine for labeling, using SacI and Eco91I restriction sites. .. For Nb-Fc constructs, pFuse-hIgG1-Fc2 plasmid (Invivogen, pFUSE-hIgG1-Fc2) -compatible restriction sites (EcoRI and NcoI) were added to the VHH genes by PCR using an EcoRI-introducing forward primer: 5′-CATGATGAATTGAGAGGTGCAGCTGGTGGAG-3′ and a NcoI-introducing reverse primer: 5′-ATCATGCCATGGCTGAGGAGACGGTGACCTG-3′ and cloned into the pFuse-hIgG1-Fc2 plasmid. .. All plasmid DNA sequences were verified using Sanger sequencing (Eurofins Genomics). .

    Article Title: Molecular Engineering of the Helminth TGF ‐β Mimetics, TGM1 and TGM4, Reveals a Novel Antagonist of TGF ‐β Signaling in Fibroblasts
    Article Snippet: .. Dimers were constructed using the pFUSE‐hIgG1‐Fc2 plasmid (InvivoGen), with or without an additional 45‐bp linker encoding 3 copies of the flexible GGGGS peptide linker. ..


    Article Title: Molecular Engineering of the Helminth <scp>TGF</scp> ‐β Mimetics, <scp>TGM1</scp> and <scp>TGM4,</scp> Reveals a Novel Antagonist of <scp>TGF</scp> ‐β Signaling in Fibroblasts
    Article Snippet: .. Dimers were constructed using the pFUSE- hIgG1- Fc2 plasmid (InvivoGen), with or without an additional 45- bp linker encoding 3 copies of the flexible GGGGS peptide linker. ..

    Plasmid Preparation:

    Article Title: NanoB 2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET
    Article Snippet: Generation of NanoLuc-tagged receptor plasmids NanoLuc-ACKR3 and NanoLuc-CXCR4 in pcDNA3.1 have been described previously.34 The pcDEF3 vector was a gift from Langer.59 The pET28a and pMEK222 vectors for periplasmic production of nanobodies in E.coli have been described previously.60,61 NanoLuc-US28 in pcDEF3 has been described previously.38 HA-US28-NanoLuc in pcDEF3 was generated by replacing BioID2 in the HA-US28-BioID2 pcDEF3 construct, which has been described previously.38 HA-CXCR4, HA-ACKR3 and HA-US28 in pcDEF3 have been described previously.62,63,64 NanoLuc-EGFR in a pNKF1-secN CMV vector was a kind gift from Promega Corporation (Wisconsin, USA), whose generation has been described previously.42. . Generation of nanobody-C-Direct and nanobody-Fc plasmids For nanobody-C-Direct constructs, VHH genes were inserted in pYQVQ11 plasmid (QVQ Holding B.V.), with a C-terminal tag containing an unpaired cysteine for labeling, using SacI and Eco91I restriction sites. .. For Nb-Fc constructs, pFuse-hIgG1-Fc2 plasmid (Invivogen, pFUSE-hIgG1-Fc2) -compatible restriction sites (EcoRI and NcoI) were added to the VHH genes by PCR using an EcoRI-introducing forward primer: 5′-CATGATGAATTGAGAGGTGCAGCTGGTGGAG-3′ and a NcoI-introducing reverse primer: 5′-ATCATGCCATGGCTGAGGAGACGGTGACCTG-3′ and cloned into the pFuse-hIgG1-Fc2 plasmid. .. All plasmid DNA sequences were verified using Sanger sequencing (Eurofins Genomics). .

    Article Title: Molecular Engineering of the Helminth TGF ‐β Mimetics, TGM1 and TGM4, Reveals a Novel Antagonist of TGF ‐β Signaling in Fibroblasts
    Article Snippet: .. Dimers were constructed using the pFUSE‐hIgG1‐Fc2 plasmid (InvivoGen), with or without an additional 45‐bp linker encoding 3 copies of the flexible GGGGS peptide linker. ..


    Article Title: Molecular Engineering of the Helminth <scp>TGF</scp> ‐β Mimetics, <scp>TGM1</scp> and <scp>TGM4,</scp> Reveals a Novel Antagonist of <scp>TGF</scp> ‐β Signaling in Fibroblasts
    Article Snippet: .. Dimers were constructed using the pFUSE- hIgG1- Fc2 plasmid (InvivoGen), with or without an additional 45- bp linker encoding 3 copies of the flexible GGGGS peptide linker. ..

    Polymerase Chain Reaction:

    Article Title: NanoB 2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET
    Article Snippet: Generation of NanoLuc-tagged receptor plasmids NanoLuc-ACKR3 and NanoLuc-CXCR4 in pcDNA3.1 have been described previously.34 The pcDEF3 vector was a gift from Langer.59 The pET28a and pMEK222 vectors for periplasmic production of nanobodies in E.coli have been described previously.60,61 NanoLuc-US28 in pcDEF3 has been described previously.38 HA-US28-NanoLuc in pcDEF3 was generated by replacing BioID2 in the HA-US28-BioID2 pcDEF3 construct, which has been described previously.38 HA-CXCR4, HA-ACKR3 and HA-US28 in pcDEF3 have been described previously.62,63,64 NanoLuc-EGFR in a pNKF1-secN CMV vector was a kind gift from Promega Corporation (Wisconsin, USA), whose generation has been described previously.42. . Generation of nanobody-C-Direct and nanobody-Fc plasmids For nanobody-C-Direct constructs, VHH genes were inserted in pYQVQ11 plasmid (QVQ Holding B.V.), with a C-terminal tag containing an unpaired cysteine for labeling, using SacI and Eco91I restriction sites. .. For Nb-Fc constructs, pFuse-hIgG1-Fc2 plasmid (Invivogen, pFUSE-hIgG1-Fc2) -compatible restriction sites (EcoRI and NcoI) were added to the VHH genes by PCR using an EcoRI-introducing forward primer: 5′-CATGATGAATTGAGAGGTGCAGCTGGTGGAG-3′ and a NcoI-introducing reverse primer: 5′-ATCATGCCATGGCTGAGGAGACGGTGACCTG-3′ and cloned into the pFuse-hIgG1-Fc2 plasmid. .. All plasmid DNA sequences were verified using Sanger sequencing (Eurofins Genomics). .


    Clone Assay:

    Article Title: NanoB 2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET
    Article Snippet: Generation of NanoLuc-tagged receptor plasmids NanoLuc-ACKR3 and NanoLuc-CXCR4 in pcDNA3.1 have been described previously.34 The pcDEF3 vector was a gift from Langer.59 The pET28a and pMEK222 vectors for periplasmic production of nanobodies in E.coli have been described previously.60,61 NanoLuc-US28 in pcDEF3 has been described previously.38 HA-US28-NanoLuc in pcDEF3 was generated by replacing BioID2 in the HA-US28-BioID2 pcDEF3 construct, which has been described previously.38 HA-CXCR4, HA-ACKR3 and HA-US28 in pcDEF3 have been described previously.62,63,64 NanoLuc-EGFR in a pNKF1-secN CMV vector was a kind gift from Promega Corporation (Wisconsin, USA), whose generation has been described previously.42. . Generation of nanobody-C-Direct and nanobody-Fc plasmids For nanobody-C-Direct constructs, VHH genes were inserted in pYQVQ11 plasmid (QVQ Holding B.V.), with a C-terminal tag containing an unpaired cysteine for labeling, using SacI and Eco91I restriction sites. .. For Nb-Fc constructs, pFuse-hIgG1-Fc2 plasmid (Invivogen, pFUSE-hIgG1-Fc2) -compatible restriction sites (EcoRI and NcoI) were added to the VHH genes by PCR using an EcoRI-introducing forward primer: 5′-CATGATGAATTGAGAGGTGCAGCTGGTGGAG-3′ and a NcoI-introducing reverse primer: 5′-ATCATGCCATGGCTGAGGAGACGGTGACCTG-3′ and cloned into the pFuse-hIgG1-Fc2 plasmid. .. All plasmid DNA sequences were verified using Sanger sequencing (Eurofins Genomics). .




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